肝臟是許多重要功能的關(guān)鍵器官，包括能量代謝、外源性化學(xué)物質(zhì)生物轉(zhuǎn)化、生理和病理?xiàng)l件下血漿蛋白合成、以及解毒作用[1]。人肝細(xì)胞是多功能細(xì)胞，包括蛋白質(zhì)合成、膽固醇合成、儲(chǔ)存蛋白質(zhì)、產(chǎn)生并分泌膽汁以及解毒作用[1-3]。HH是體外培養(yǎng)用來(lái)研究肝功能、新陳代謝和肝病非常好的模型。了解肝臟的分子機(jī)制可能有助于闡明治療肝臟疾病的新療法。
Sciencell研究實(shí)驗(yàn)室的臍靜脈內(nèi)皮細(xì)胞是從人肝臟分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度超過(guò)5×10^6/ml。細(xì)胞特性經(jīng)白蛋白、cytokeratin-18 and vimentin免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。HH不能擴(kuò)大培養(yǎng)或者產(chǎn)期培養(yǎng)，因?yàn)樗荒茉鲋场?

推薦培養(yǎng)基：肝細(xì)胞培養(yǎng)基 (HM, Cat. #5201)

貨號(hào)	5200
產(chǎn)地	美國(guó)
縮寫	HH
規(guī)格	1 x 10^6 /1ml
用途	科研
儲(chǔ)存	液氮
運(yùn)輸	干冰
關(guān)鍵字	人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH 人肝細(xì)胞 HH
公司簡(jiǎn)介	合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國(guó)代理商，專業(yè)經(jīng)營(yíng)實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無(wú)血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無(wú)血清培養(yǎng)基的研究和開(kāi)發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對(duì)購(gòu)買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到終售后的確保項(xiàng)目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得上等服務(wù)！
聯(lián)系方式	何經(jīng)理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898， E-mail：info@qingbio.com http://m.huangyuansheng.cn QQ:514713116

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8.) Yeon JH, Na D, Park JK. (2010) "Hepatotoxicity assay using human hepatocytes trapped in microholes of a microfluidic device." Electrophoresis. 31: 3167-74.

9.) Braconi C, Meng F, Swenson E, Khrapenko L, Huang N, Patel T. (2009) "Candidate therapeutic agents for hepatocellular cancer can be identified from phenotype-associated gene expression signatures." Cancer. 115: 3738-48.

10.) Huang Y, Song N, Ding Y, Yuan S, Li X, Cai H, Shi H, Luo Y. (2009) "Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis." Cancer Res. 69: 7529-37.

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Question:

Dear Customer Service, I am Zhonghui Zhang who is working in Dr. Wen-Shu Wu’s lab at the University of Illinois at Chicago (UIC). We previously ordered your HH cells and HH medium 09/29/2008 in Maine Medical Center Research Institute (Our lab moved from MMCRI to UIC in 2013). We would like to order a new HH cells from your company, but we don’t want to order this cell from the same donor. Would you please tell me whether the cells were from different donors? By the way, would you please give us some information about previous HH cells (such as age, gender, race and etc.)? Thank you so much! Zhonghui Zhang, Ph.D Section of Hematology/Oncology, Department of Medicine University of Illinois at Chicago 909 S Wolcott Ave, Room 5140 Chicago, IL 60612 Phone: 312-996-2324 (office) or 207-420-5603 (cell)

Answers:

Posted by Manoj Sharma on Thursday, January 7, 2016

hello,

We need lot number to check donor information. And yes we have other lots from different donors. thanks for your interest

SCIENCELL TEAM

Question:

Can you provide more detail on the source of these cells and general characteristics other than "HH from ScienCell Research Laboratories are isolated from human liver"? Derived from healthy liver tissue, any particular liver site for selection of cellular material, how derived, normal karyotype?

Answers:

Posted by Karishma Shah on Thursday, August 27, 2015

Hi Jeff,

Thanks for asking! Human Hepatocytes are isolated from healthy liver tissue. We have donor information for each lot, if you would like specific information please email sales@sciencellonline.com or call us at 760-602-8549. Due to tissue limitation, there is no particular site in the liver we get cells from. We use the entire liver tissues we obtain. Please let us know if you have any more questions. Thank you!