淋巴系統(tǒng)是免疫系統(tǒng)的重要部分，與血管系統(tǒng)共同作用來保持組織的內(nèi)環(huán)境穩(wěn)定。淋巴系統(tǒng)從組織中得到液體和大分子物質(zhì)并將其返回血液循環(huán)，因此，淋巴系統(tǒng)在調(diào)節(jié)組織中液體，蛋白質(zhì)和壓力平衡方面起著重要作用。盡管淋巴毛細(xì)管內(nèi)皮細(xì)胞與血管內(nèi)皮有許多相似的特性，但由于結(jié)構(gòu)不同，又各具特點(diǎn)。淋巴毛細(xì)管缺少壁細(xì)胞且以基底膜不完整或缺失為特征。淋巴內(nèi)皮的特征是包含大量的內(nèi)褶和細(xì)胞質(zhì)囊泡，并且具有交錯(cuò)的細(xì)胞間連接。淋巴毛細(xì)管*顯著的特征是與間質(zhì)的一體化，間質(zhì)通過細(xì)微的彈性纖維與細(xì)胞外基質(zhì)相連。
淋巴內(nèi)皮細(xì)胞提取于人淋巴結(jié)組織，**代凍存。每管含有細(xì)胞數(shù)>5×10^5cells/ml，此細(xì)胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基(ECM, Cat. #1001)
|
貨號(hào)
|
2500
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HLEC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
|
關(guān)鍵字
|
人淋巴內(nèi)皮細(xì)胞 HLEC 人淋巴內(nèi)皮細(xì)胞 HLEC 人淋巴內(nèi)皮細(xì)胞 HLEC 人淋巴內(nèi)皮細(xì)胞 HLEC 人淋巴內(nèi)皮細(xì)胞 HLEC 人淋巴內(nèi)皮細(xì)胞 HLEC
|
公司簡(jiǎn)介
|
合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國代理商，專業(yè)經(jīng)營實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無血清培養(yǎng)基的研究和開發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對(duì)購買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項(xiàng)目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)！
|
|
聯(lián)系方式
|
何經(jīng)理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://m.huangyuansheng.cn QQ:514713116
|
1.) Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH. (2014) 'Metabotropic Glutamate Receptor-1 as a Novel Target for the Antiangiogenic Treatment of Breast Cancer.'
2.) Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH. (2014) "Metabotropic Glutamate Receptor-1 as a Novel Target for the Antiangiogenic Treatment of Breast Cancer."?PloS one. 9: e88830.
3.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-? in human cholangiocarcinoma QBC939 cells promote tumor progression." PLoS One. 8: e62024.
4.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) 'Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation.'
5.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) "Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation."?BioMed res int. 2013: 208912.
6.) Qiu Y, Chen Y, Fu X, Zhang L, Tian J, Hao Q. (2012) "HMGB1 promotes lymphangiogenesis of human lymphatic endothelial cells in vitro."?Med Oncol. 29: 358-63.
7.) Bouta EM, McCarthy CW, Keim A, Wang HB, Gilbert RJ, Goldman J. (2011) "Biomaterial guides for lymphatic endothelial cell alignment and migration."?Acta Biomater. 7: 1104-13.?
8.) Hu J, Ye H, Fu A, Chen X, Wang Y, Chen X, Ye X, Xiao W, Duan X, Wei Y, Chen L. (2010) "Deguelin--an inhibitor to tumor lymphangiogenesis and lymphatic metastasis by downregulation of vascular endothelial cell growth factor-D in lung tumor model."?Int J Cancer. 127: 2455-66.
9.) Zhuang Z, Jian P, Longjiang L, Bo H, Wenlin X. (2010) "Altered phenotype of lymphatic endothelial cells induced by highly metastatic OTSCC cells contributed to the lymphatic metastasis of OTSCC cells."?Cancer Sci. 101: 686-92.?
10.) Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ. (2009) "Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model."?Int J Cancer. 124: 2709-18.?
11.) Thapa N, Hong HY, Sangeetha P, Kim IS, Yoo J, Rhee K, Oh GT, Kwon IC, Lee BH. (2008) "Identification of a peptide ligand recognizing dysfunctional endothelial cells for targeting atherosclerosis."?J Control Release. 131: 27-33.?
12.) Yoon CM, Hong BS, Moon HG, Lim S, Suh PG, Kim YK, Chae CB, Gho YS. (2008) "Sphingosine-1-phosphate promotes lymphangiogenesis by stimulating S1P1/Gi/PLC/Ca2+ signaling pathways."?Blood. 112: 1129-38.
13.) Zhuang Z, Jian P, Longjiang L, Bo H, Hongwei Z. (2008) "Identification of oral cancer cell-induced changes in gene expression profile of lymphatic endothelial cell."Cancer Invest. 26: 1002-7.
Question:
Can you tell me the FVIII antibody used to test these cells. What company and catalogue # please.
Answers:
-
Posted by Karishma Shah on Tuesday, October 18, 2016
Dear Colleen,
We use Anti-von Willebrand factor antibody (otherwise known as Factor
VIII related antigen) from Sigma-Aldrich. The catalog number is F3520.
Question:
Do these express podoplanin
Answers:
-
Posted by Manoj Sharma on Thursday, January 14, 2016
Our quality control department does not test for podoplanin
expression in Human Lymphatic Endothelial Cells. We do test for
endothelial cell markers CD-31 and Factor VIII.
